Reporter

Part:BBa_K1316016:Design

Designed by: Joan Cortada Garcia   Group: iGEM14_TU_Delft-Leiden   (2014-09-29)


constitutively expressed eGFP under the control of J23110 Anderson promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

eGFP was desired to be constitutively expressed. The sequence was screened for illegal iGEM restriction sites


Source

eGFP was PCRed out a conventional plasmid containing the coding sequence for this reporter gene. The constitutive promoter is the iGEM J23110 Anderson promoter

References